FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences359993100
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATATAAATGCGTAGGGGTTTT3667550.10187834155710206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAGAG1253000.018.0033951
ACGCCGA1912550.017.9197461
GTATGAA1423900.017.8395548
ACTGAAC1077450.017.8075791
CCACCTA1998150.017.762171
ACGAGGA1289950.017.7191721
GGGAGAT987600.017.699751
GGGACCT1378600.017.6483461
TATGAAT1190700.017.6394469
CCGTGGA1192100.017.6234841
AAACCTG1407500.017.588031
TAAATGA703700.017.5650799
GGGAATG1082550.017.5528981
CGGAGCT1286900.017.5322151
AAGGAAG817450.017.53160310
ATGAATG1238900.017.5226610
CTAAGAC1316750.017.5222171
CCGGAAA725500.017.51336910
CGTGAGC1341900.017.4811631
AGAAACC1267150.017.4710778
Produced by FastQC (version 0.11.5)