FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences359993100
Sequences flagged as poor quality0
Sequence length8-98
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7422580.20618672969009685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1968250.047.9732179
AAGCAGT2424500.042.9277271
GTGGTAT2269400.040.7112466
GGTATCA2318350.040.5796558
TGGTATC2455650.037.4781157
ATCAACG1865150.032.1059810-11
CAACGCA2003400.030.844112-13
AGCAGTG3557900.029.3985842
TCAACGC2013450.028.16341412-13
ACGCAGA2280200.026.48912614-15
AGAGTAC1977050.025.24951618-19
CAGTGGT3809500.024.806724
AACGCAG2381650.024.5295514-15
GCAGTGG4233450.024.1747653
ACGACGC263300.024.0614835
CGCAGAG2489700.022.46994416-17
CTACACG289300.022.3622321
AGTGGTA4587900.020.931875
CAGAGTA2353350.020.2191618-19
GTACTTT2368500.018.55827522-23
Produced by FastQC (version 0.11.5)