FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences338658163
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGTCTGTCTTGCGCCGGTCCA16105050.4755547557848177No Hit
TTCGGTCTTGATTAATGAAAACATTC6833000.20176687723898157No Hit
CATGCCTGTGTTGGGTTGACAGTG4804810.14187787347089578No Hit
CGTTCGTTATCGGAATTAACCAGAC4223450.12471130069881114No Hit
ACGCCAGAGTCTCGTTCGTTATCGG3702680.10933384765333413No Hit
AGTTGGTTGATTGTAGATATTGGGCT3491380.10309451776008129No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGAAA2292250.018.8960236
ATAGCTA3473600.018.719546
GTATGAA2310550.018.6589668
CTGGTCT7218300.018.621111
CGCGAAA1609400.018.6094610
TCTAGAA2353050.018.605775
TAGCTAG3597850.018.4983067
AGCTAGG3553900.018.4870538
CTACAAA1764750.018.4447825
GCTACAA1796550.018.4292724
AGAAACC2789450.018.4182668
GAGTAGA3521750.018.40452610
GATAGCT3539800.018.3927785
TGACAGA1840700.018.3923345
TACGGAT1854400.018.3810561
CATATTC3053550.018.3587741
TATGAAA1029050.018.3562229
AGATAAT2014350.018.3528759
TACTAGA1464200.018.3511494
TATTCCA2953200.018.3475593
Produced by FastQC (version 0.11.5)