FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences338658163
Sequences flagged as poor quality0
Sequence length8-98
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGT5790810.17099277775270988No Hit
GCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCT5103620.15070122493991087No Hit
GCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTC4731810.13972230753522394No Hit
GTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAG4356410.12863738353178278No Hit
GGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGT4259930.1257884931006373No Hit
GTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAA3868550.11423170685538739No Hit
GCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCT3841140.11342233613899336No Hit
GTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGG3728690.11010187874904405No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3618190.10683900154504766No Hit
GTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTA3617320.10681331192362252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1464850.051.669129
GGTATCA1607250.046.065718
AAGCAGT2005300.045.0334431
TGGTATC1621400.042.6305477
GTGGTAT1709050.041.601286
CAACGCA1828750.031.78025212-13
ATCAACG1727750.031.68568410-11
TCAACGC1758700.028.80062512-13
ACGCAGA2036500.028.16305514-15
AGCAGTG3177550.028.145052
AGAGTAC1708800.024.418918-19
GCAGTGG3450700.024.2903393
AACGCAG2389000.023.21232214-15
CGCAGAG2225300.023.06344816-17
ACGACGC252750.022.1860145
CAGAGTA1898500.021.1974218-19
CAGTGGT3420350.021.1906934
CTACACG294500.019.5845171
GCAGAGT2255250.019.09372516-17
GTACTTT1545850.019.06367122-23
Produced by FastQC (version 0.11.5)