FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences350746572
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGGCTGTTAATTGTCAGTTCAGTG4940750.1408638143439931No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGGA1308150.018.1647361
CCGGGAT1474200.018.0555521
GACCAAT1299050.017.6621421
CAATGAA697500.017.5233388
CTCGAAA806800.017.394241
GACTAAC807050.017.3912981
ATGCGAT1120300.017.3597871
TGCCAAA998050.017.2196221
CTGCCTA1440300.017.145651
ATGAATG1065400.017.12697810
TGCGGGT1728200.017.0837331
ACGCCGA1312450.017.0652031
CGCTGGA875800.017.0493411
AGGGTGA1786350.016.9861951
CTCAGAA1090400.016.9469641
TCTGGAA1026800.016.946061
CCCGAAA518750.016.91734110
ACACCCT1871800.016.9167841
GAATGAA841100.016.8832131
GATGAAA638050.016.851361
Produced by FastQC (version 0.11.5)