FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences363972828
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATATAAATGCGTAGGGGTTTT6839920.18792391832062805No Hit
CCACCTACTAAGAACGGCCATGCACC6686020.18369558070417277No Hit
TGTTCCGTTGGTCAAGTTATTGGATC5536630.1521165750318043No Hit
CGTTCGTTATCGGAATTAACCAGAC4539970.12473376171915779No Hit
ATGAGGGTAATAATGACTTGTTGGTT4219210.11592101595012472No Hit
ACCTTTAAGTTTCAGCTTTGCAACC3856810.10596422873632753No Hit
CAGCCGAGTTCCTTTTACTTTTTTT3838620.10546446615514937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTAA2031950.018.6470459
CGGAGCT2407250.018.6359631
ATGGGAG2371000.018.6231691
TGGGAGA1897800.018.5611422
TTGGCTA3023600.018.512112
AAGAATG1666500.018.5063957
TGGCTAG2949350.018.4112383
TACGGTA2263600.018.3786351
AGACTAG2593200.018.3739058
TAAGAAT1720850.018.3625896
ACTAGAT2546900.018.3096410
GACTAGA2552450.018.3054649
TGGCCAG2254100.018.2907431
GGAGGTA2935600.018.28311510
GTAAGAA1592250.018.1997365
CAGACGA1163900.018.1804457
CCTAAGA1371000.018.159394
TAGACTA2381750.018.1576187
AGAGAAC1370650.018.154058
CGGTACA2740050.018.1466123
Produced by FastQC (version 0.11.5)