FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences363972828
Sequences flagged as poor quality0
Sequence length8-98
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGT6329130.1738901784173845No Hit
GCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCT5778920.15877339063343487No Hit
GCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTC5566390.15293421848512273No Hit
GTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAG5246920.1441569149222315No Hit
GGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGT5237860.14390799524188658No Hit
GTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAA4895610.13450482078294043No Hit
GTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTA4552200.12506977581304504No Hit
CTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCA4076300.1119946239503351No Hit
GCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCT3991530.10966560393898415No Hit
GTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTT3854460.10589966347707691No Hit
GTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGG3836590.1054086927609882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1101050.044.916769
GGTATCA1306550.037.328498
AAGCAGT1727500.035.879231
GTGGTAT1360000.035.2341656
TGGTATC1476650.031.6599037
CAACGCA1299300.030.14788812-13
ATCAACG1230050.029.88352810-11
TCAACGC1302050.026.39293312-13
ACGCAGA1565850.024.7987914-15
AGCAGTG2802000.021.785632
ACGACGC262250.020.377125
AACGCAG1860450.020.28497714-15
AGAGTAC1361000.019.92652518-19
CGCAGAG1802250.019.57130216-17
CAGAGTA1451400.018.21211818-19
GCAGTGG3212800.017.7595773
CTACACG316500.017.4093781
GCAGAGT1757300.016.3309116-17
CAGTGGT3168900.015.8572674
TACACGA347300.015.6992312
Produced by FastQC (version 0.11.5)