FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352471070
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTGGTTGATTGTAGATATTGGGCT4464230.12665521740550223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGAAA1220450.018.511111
CGCCGAG1635900.018.3616872
ACGCCGA2594200.018.3234541
CTCGAAA1010750.018.1457651
TCGGAAA1213050.018.044498
GGAAATA1031900.017.8692710
TAGAGAG967350.017.7481425
GACTAAC1077700.017.7285161
GCCGAAG794800.017.5888353
ACTGAAC1046550.017.5203171
GGACGAA697250.017.472019
CATGGAA956500.017.4673678
AAGAAGG1088100.017.4605887
GACTACA1682000.017.4559751
AGAGAAG1034550.017.4512213
CGCTGGA993900.017.4408071
AGGACGA701750.017.352788
GAAGTGG1209700.017.3340236
CCCGACA1035050.017.31992310
TTCACGA1135350.017.3057047
Produced by FastQC (version 0.11.5)