FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352471070
Sequences flagged as poor quality0
Sequence length8-98
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7590660.21535554676870358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2942650.048.024911
GTATCAA2633350.044.430429
GGTATCA3051000.038.4218188
TGGTATC2980500.038.4165157
GTGGTAT3147150.036.835416
AGCAGTG4076750.035.2982142
AGTGGTA3815650.031.3014285
CAGTGGT3852200.031.1875024
ATCAACG3001200.027.57173310-11
GCAGTGG5356750.026.342453
CAACGCA3482950.025.93623512-13
ACGCAGA3630250.024.33749414-15
TCAACGC3239900.023.73336212-13
AGAGTAC2748350.023.61184918-19
CTACACG318300.021.7176231
AACGCAG3879700.021.28562414-15
CGCAGAG4173950.019.20707316-17
CAGAGTA3340350.018.20917918-19
GCAGAGT3772250.018.0294916-17
GTACTTT2691300.017.93627522-23
Produced by FastQC (version 0.11.5)