FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences350926431
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATGAA1132900.018.5323121
CTCGAAA1114950.018.4954281
AAACCTG1671150.018.2021
GATGAAA849800.017.8487661
CCGGGAT1253600.017.8224751
TCGGTAA1507800.017.7200261
GAGCTAA1034250.017.6975847
ACACTGA1474450.017.538311
TAAACCG1194600.017.4944171
GACCAAT1097750.017.4658011
CGTGAGA856800.017.40807510
CCACCTA2206750.017.3808161
CGTTGGG2261950.017.3636231
TACACGA1124600.017.3573041
TGGCCAG1387200.017.3550741
AATGAAC833600.017.3051762
TTTAGAT930400.017.2994695
CGATTGA1141600.017.276471
ACTTTAG1266450.017.2481843
GATTCAG1172250.017.2199331
Produced by FastQC (version 0.11.5)