FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences350926431
Sequences flagged as poor quality0
Sequence length8-98
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4037880.1150634333382543No Hit
GTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGT3731350.10632855408944675No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1890500.045.661369
AAGCAGT2563450.041.3180581
GTGGTAT2034150.038.9172026
GGTATCA2188600.037.9591948
TGGTATC2192750.035.5909087
CAACGCA2449600.029.75240112-13
ATCAACG2332300.029.46958410-11
AGCAGTG3437800.029.3897342
TCAACGC2357700.026.9632812-13
ACGCAGA2707900.026.8549914-15
AACGCAG2806500.024.32215314-15
AGAGTAC2102900.023.91433118-19
GCAGTGG3940850.023.3590873
CGCAGAG2915750.022.37385416-17
CAGTGGT3848500.021.3302174
GCAGAGT2560350.020.44978516-17
CAGAGTA2381000.020.32713118-19
ACGACGC287350.020.2462025
CTACACG311650.019.2358471
TATCAAC2672850.018.6595910-11
Produced by FastQC (version 0.11.5)