FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences359103375
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCCGCAGGCTCCACTCCTGGTGGT5438760.15145388149025332No Hit
CGTTCGTTATCGGAATTAACCAGAC4374700.12182285950389635No Hit
CCACCTACTAAGAACGGCCATGCACC4268250.11885853203134056No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAAAGG1659250.018.34870710
CCGAGAA1119700.018.2912655
CCACCTA2177900.018.2748761
GACCAAT1635050.018.2621521
ATAAGAG1296800.018.1947481
TCCGAGA1112350.018.133224
ACGATAC1925650.018.0487581
TAGAAAG868850.017.990579
CAATGGA1922350.017.9707268
ACAAACA1362000.017.9196432
AATGTAA1283750.017.8840269
AGACTAG1199450.017.8812948
GACTAAC952450.017.7992671
CTGATAG1017550.017.7820951
AGGGATG1860700.017.7744121
TGCACGA1171250.017.7662129
AATAACA1054450.017.759033
GACCGGA973400.017.7463659
TATCGAA1178450.017.70324110
GATGAGA1566950.017.6995434
Produced by FastQC (version 0.11.5)