FastQCFastQC Report
Fri 22 Apr 2022
EGAF00005798453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences359103375
Sequences flagged as poor quality0
Sequence length8-98
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1404850.039.2976389
AAGCAGT2045050.034.309691
GGTATCA1692950.031.929818
ATCAACG1427150.028.89722410-11
GTGGTAT1808150.028.6937686
CAACGCA1597400.027.97995412-13
TGGTATC1836450.027.9036317
TCAACGC1512850.025.37180512-13
AGCAGTG2981600.023.2046242
ACGCAGA1966700.022.31225614-15
AGAGTAC1425700.021.36881318-19
AACGCAG1944550.021.30415214-15
AGTGGTA2774500.019.5974395
CTACACG314050.018.6549931
CGCAGAG2131700.018.22253816-17
ACGACGC323100.017.9663375
CAGTGGT3161650.017.2372324
TACACGA371800.015.6262292
GAGTACT1763750.014.72134120-21
GCAGTGG4545350.014.5531913
Produced by FastQC (version 0.11.5)