FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334890223
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACCTACTAAGAACGGCCATGCACC6693020.19985713348221576No Hit
AAGCCGCAGGCTCCACTCCTGGTGGT6356440.18980667584314637No Hit
CTGCGGAGGAGAATGTTTTCATGTT5745580.17156607166761031No Hit
GTCTCGTTCGTTATCGGAATTAACC4892020.146078316535386No Hit
ATGAGGGTAATAATGACTTGTTGGTT4536860.1354730502239834No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACCT2577300.018.7507151
AGAAAGG2454450.018.73137710
TAGAAAG2376000.018.7142439
GAATGAA1134200.018.628341
GGCGACT1900100.018.5764641
GGACCTA2267250.018.5661662
AAGAAAA886750.018.4996262
GAAAAGA779600.018.4859624
AGAAAAG888300.018.415973
AAGAAAC1477600.018.329837
CGATTGA1517500.018.3215471
CCCAACG2429850.018.3169069
ATAAAGA862800.018.3169044
GAATAAA1070750.018.3069932
AATAAAG1071000.018.2949493
AAAAGAG639900.018.2939115
GTCTAAA1958250.018.2747848
CTGCGGA2798100.018.2730221
TGAGAAT1754700.018.2692286
GTAGAAA2449600.018.2368558
Produced by FastQC (version 0.11.5)