FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334890223
Sequences flagged as poor quality0
Sequence length8-98
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6136070.18322631055132355No Hit
GTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGT3585550.10706642815308466No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2867900.049.758311
GTATCAA2569100.047.178869
GGTATCA2688250.044.4077348
TGGTATC2650600.042.7906047
GTGGTAT2752600.041.3044136
AGCAGTG3850750.036.9776762
ATCAACG3035200.030.4961110-11
CAACGCA3282200.029.5666712-13
CAGTGGT3962600.029.1514194
ACGCAGA3389850.027.83720414-15
GCAGTGG4967950.026.8576053
TCAACGC3140900.026.84711812-13
AGTGGTA4385300.026.5601335
AGAGTAC2586000.026.1647818-19
AACGCAG3531300.024.7544114-15
CGCAGAG3643350.022.85959816-17
CTACACG282450.022.7014031
GCAGAGT3290950.021.42838316-17
CAGAGTA3008600.021.07174718-19
ACGACGC317800.019.7345815
Produced by FastQC (version 0.11.5)