FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences336879977
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCGTTCGTTATCGGAATTAACC7637070.2267000273512842No Hit
GCAATCATACTCCCCCCGGAACCCA3712110.11019087667534483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATG3283450.019.0219141
GAGGATA1977050.018.7098227
TAGAGAA1665600.018.5971155
GATGAAA1415550.018.5374161
TCGAGGA1549550.018.5317552
CTGATGA2602850.018.4482062
AGAGAAA1116150.018.4002976
ACCCACT2543200.018.3908751
TGATGAG2610700.018.3575573
TGACTAG1657450.018.343491
GGATATA2111750.018.3236359
CCTTGAG2060900.018.3062463
GGACGCA1568650.018.261572
GGAGGAT1727350.018.2186456
TGAGGAT2034350.018.1872086
AGGATAT2129850.018.165578
ACCGGAG1372050.018.1544443
TGCCAAA1261200.018.1516111
AGGCGAA840250.018.1457110
CGAATCA2287050.018.14314
Produced by FastQC (version 0.11.5)