FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences336879977
Sequences flagged as poor quality0
Sequence length8-98
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3772890.11199508007565555No Hit
GCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTC3370550.10005195411183491No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2599850.045.454831
GTATCAA2088850.044.5144239
GGTATCA2225000.040.9861228
TGGTATC2260350.038.7981957
GTGGTAT2379950.037.3851976
AGCAGTG3304550.035.3112342
ATCAACG2481950.029.30605910-11
CAACGCA2765550.028.27389112-13
TCAACGC2574550.025.92228712-13
AGTGGTA3719700.024.4576765
ACGCAGA3128400.024.2587614-15
AGAGTAC2171000.023.6008418-19
AACGCAG2988550.023.58973314-15
GCAGTGG4692500.023.3295633
CAGTGGT4098300.022.3846744
CTACACG290050.021.644541
CGCAGAG3077300.021.58796916-17
ACGACGC320850.018.821155
CAGAGTA2607700.018.7417918-19
TACACGA345400.017.6718672
Produced by FastQC (version 0.11.5)