FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343266303
Sequences flagged as poor quality0
Sequence length8-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCCGCAGGCTCCACTCCTGGTGGT3520160.10254895307914916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA1706800.017.9787968
AGAAAGG1149400.017.96653410
CGCTGGA1243050.017.804411
CTCGAAA851250.017.7355631
TGCCAAA1396750.017.6799661
CTGATAG1146850.017.645451
CTAAACA888650.017.34515410
CCCTCCT3189700.017.3189951
ACTGAAC861650.017.2299331
ATAGACC1136150.017.2213921
CGATTGA933850.017.1762031
AACGAAA640150.017.12243310
GATGAAA774100.017.0972121
AAAGATG1466100.017.090581
CGAGGAA513450.017.0033282
AAACCTG1232200.016.9345341
CATAAGA745100.016.9103644
TGACTAG908950.016.8686941
TCAATGG1466050.016.855847
GAATGAA628150.016.8435021
Produced by FastQC (version 0.11.5)