FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343266303
Sequences flagged as poor quality0
Sequence length8-98
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3512550.10232725931155556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1831400.041.060269
AAGCAGT2510050.036.4320221
GGTATCA2123100.034.292898
TGGTATC2157100.032.050127
GTGGTAT2253000.030.9137696
ATCAACG1978250.029.26900510-11
CAACGCA2342150.027.49518812-13
AGCAGTG3211550.027.4850852
TCAACGC2161800.024.92263212-13
AGTGGTA2888050.024.7675085
ACGCAGA2641000.023.60583714-15
AACGCAG2590000.022.58861514-15
CAGTGGT3256350.022.0329554
AGAGTAC1978850.021.2738118-19
TATCAAC2174950.019.19533310-11
CGCAGAG3040050.018.46084816-17
CAGAGTA2476450.016.09059518-19
GCAGTGG5334850.015.9964073
GTACTTT2096550.014.47114722-23
GCAGAGT3461650.013.17482216-17
Produced by FastQC (version 0.11.5)