FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345275624
Sequences flagged as poor quality0
Sequence length8-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAA1034000.018.044161
CAATGAA881200.017.8736488
TACGGAT1595300.017.724661
GATGCTA1821050.017.7054841
ATAGACC1263650.017.50311
CCGGTAG1554950.017.4445531
ACTGATG1150350.017.4236471
GAAATGA881650.017.4133471
CGCTGGA1228000.017.324891
TAACAGA689500.017.3083864
AACGCGA1208600.017.30664310
CCACCTA1742800.017.2846341
GCCGACA881350.017.0845093
ACTGCGA987650.017.0205422
TCTGGAA885500.016.9932461
GGAAAGC782200.016.9776941
CAATGGA948350.016.9670338
AGCTGAA760650.016.9564638
ATCCGCA1125850.016.9530987
ACGCCGA1394300.016.9396061
Produced by FastQC (version 0.11.5)