FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345275624
Sequences flagged as poor quality0
Sequence length8-98
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3956210.11458121352928174No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1554750.045.0491839
GGTATCA1920350.036.08968
AAGCAGT2504100.035.811521
TGGTATC2143700.031.389137
ATCAACG1633850.031.23605210-11
GTGGTAT2273450.030.0030636
CAACGCA1982350.028.72363712-13
AGCAGTG3252600.027.3285262
TCAACGC1835200.026.24122412-13
AGTGGTA2807000.025.2552415
ACGCAGA2297250.024.16228314-15
AACGCAG2252700.023.28477514-15
CAGTGGT3082600.023.0826154
AGAGTAC1828800.020.81438318-19
CGCAGAG2660950.019.11050616-17
TATCAAC2017150.018.62961610-11
GCAGTGG5286650.016.4985263
CAGAGTA2265850.015.939754518-19
GTACTTT1943050.014.67776222-23
CTACACG279400.012.3693721
Produced by FastQC (version 0.11.5)