FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345987243
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCCGCAGGCTCCACTCCTGGTGGT4557980.13173838319813427No Hit
CCACCTACTAAGAACGGCCATGCACC4374090.1264234473523638No Hit
ACGGGCTGTTAATTGTCAGTTCAGTG4191840.12115591209818104No Hit
GTCTCGTTCGTTATCGGAATTAACC3817660.11034106248824904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA2504500.017.9873898
CCACCTA1731900.017.6608981
GACTAAC978500.017.6108631
GTGATAA1150500.017.590628
ATGCGAT964850.017.3502221
GAATGAA900450.017.1919631
TCGGTAA1161550.017.1897071
TACGGAT1232600.017.1607461
GGGACCT1153300.017.1461931
CAATGAA719400.017.0442228
ACGGGCT2103350.017.0385951
CATCGAA1232000.016.9465851
CTAATGG1136000.016.9246711
CCGGGAT910950.016.9199261
CACCGAA702050.016.9106758
AGTGATA878500.016.8906447
ATTGAGA463650.016.7947144
TTGTAGG1464900.016.7769891
AGAAGAT866100.016.7691088
CCCTCCT1953950.016.7509651
Produced by FastQC (version 0.11.5)