FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345987243
Sequences flagged as poor quality0
Sequence length8-98
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGT6659890.19248946701771893No Hit
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA5247320.15166223917683577No Hit
GCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCT4915260.14206477549231492No Hit
GTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGG4399210.1271494856820487No Hit
GGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCT4364070.1261338412988828No Hit
GCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCT4041390.11680748587600381No Hit
GTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAG3991850.11537564117645807No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3902530.11279404310291291No Hit
GGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACG3861020.1115942878853484No Hit
GCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAAC3644520.10533683173977602No Hit
GGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATA3519050.10171039745531889No Hit
GCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTC3476360.10047653693405105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1340250.047.433159
GTGGTAT1476050.041.2215236
GGTATCA1555000.040.4675148
TGGTATC1533600.039.097657
AAGCAGT2015350.035.3957181
CAACGCA1400900.031.21538212-13
ATCAACG1384150.030.68930810-11
TCAACGC1420400.028.3469712-13
AGCAGTG2792000.025.5112932
ACGCAGA1715400.024.63964814-15
AGAGTAC1431900.022.59533918-19
ACGACGC265850.022.0728845
CAGTGGT2925100.021.2807084
GCAGTGG3278250.021.2076173
AACGCAG1987300.021.1521414-15
CGCAGAG1954550.019.85323516-17
CTACACG311050.018.9781511
CAGAGTA1682750.018.5822218-19
TATCAAC1948050.017.4292910-11
GCAGAGT1972200.017.29954116-17
Produced by FastQC (version 0.11.5)