FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378979352
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATATAAATGCGTAGGGGTTTT5068800.13374871146014308No Hit
CGTTCGTTATCGGAATTAACCAGAC4554780.12018543954869605No Hit
ACGGGCTGTTAATTGTCAGTTCAGTG4458590.11764730654771927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGACA976900.018.0275696
TGAAGAC960650.017.7419625
AACGAAA832900.017.68407410
ACGCCGA1948750.017.6565841
GCCGAAG903700.017.4602993
GCCTCTA2615450.017.4585231
CAATGGA1909700.017.4322178
CTCGAAA748000.017.4015661
CTGGAAA912900.017.396762
GTGCATA1393650.017.3762841
TCTGGAA1205600.017.3580951
CATCGAA1343150.017.3394471
TATGCCC1987750.017.3181381
AGACTAA733650.017.3067478
CGCTTCA1961900.017.2850631
GACCTGG1618400.017.2726861
CGCTTAG1318950.017.2580499
GTATGAA1185250.017.249318
ACGAGGA791350.017.2400951
ATGGGAG1228250.017.1970141
Produced by FastQC (version 0.11.5)