FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378979352
Sequences flagged as poor quality0
Sequence length8-98
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4498100.11868984355643734No Hit
GGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGT3917570.10337159476698878No Hit
GTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAA3836410.10123005329324643No Hit
GTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAG3816980.10071736045398061No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1388600.047.0471579
GGTATCA1673400.038.3644758
TGGTATC1734050.035.808187
GTGGTAT1856200.034.5248646
AAGCAGT2192200.033.9914321
ATCAACG1440450.030.31639310-11
CAACGCA1622150.028.42847812-13
TCAACGC1550550.026.2963712-13
AGCAGTG3027550.024.5076392
ACGCAGA1944150.023.66945314-15
AACGCAG2074650.021.13104614-15
CAGTGGT3160050.020.7067474
AGAGTAC1785150.020.35022418-19
AGTGGTA3562200.018.9865635
CGCAGAG2331900.018.04292716-17
CTACACG271700.017.3654161
CAGAGTA2030100.017.18544418-19
GTACTTT1746300.016.84099822-23
GCAGTGG4357800.016.7243983
ACGACGC277000.016.5807935
Produced by FastQC (version 0.11.5)