FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340223695
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGGA1657250.018.2336311
GAATGAA892350.017.7372061
CACGAAA1140900.017.6504368
GAAATGA1018850.017.5609231
CCACTAC1569450.017.5601841
GCAGTTA1242900.017.5557561
CTCGAAA737250.017.5131241
ACACTGA1512000.017.4238171
GCCGAAG831300.017.407953
GGGAGAT1205850.017.407341
AGAAACC1391700.017.4061138
ATAGACC929900.017.3242861
CCGTGGA1229850.017.3017981
TTTGTCA1660850.017.1816751
GACTAAC809750.017.1764321
AGAGCGA842800.017.1253971
TTAGGCA1394100.017.081411
ACTGAAC778750.017.0306841
GGCCCTA1019050.017.02019110
ATCGAGT1119700.016.9886151
Produced by FastQC (version 0.11.5)