FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences292636035
Sequences flagged as poor quality0
Sequence length8-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGAAA809850.018.0768871
GAATGAA810600.017.8142621
GACTAAC1052300.017.8109971
AAGAAAA353600.017.7789022
TGCCAAA826550.017.5707821
CAAGAAA599750.017.5124131
ACGAGCC1370750.017.4906441
CTCGAAA632650.017.4174421
AGACTAG975800.017.344758
CAATGGA1509150.017.2505258
ATGCGAT875700.017.2471661
CACCGAA594200.017.2044098
AAACCTG1047350.017.185741
TTGACTT1354900.017.033241
GAAAAGA337850.017.026614
TCAACGA1326300.017.0054781
CAATGAA485500.016.9929548
TACAAGA515300.016.9271974
GAATAGA631600.016.8804232
GGGACCT795900.016.848931
Produced by FastQC (version 0.11.5)