FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences292636035
Sequences flagged as poor quality0
Sequence length8-98
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1086000.038.7112549
GGTATCA1219750.033.371968
AAGCAGT1537150.032.2041631
TGGTATC1304750.029.8187167
GTGGTAT1369900.028.6043576
ATCAACG1045450.027.67897610-11
CAACGCA1192750.025.5401112-13
TCAACGC1151000.023.72737112-13
AGCAGTG2114900.022.8549182
CTACACG170000.022.547211
AGTGGTA1964800.020.3931835
ACGCAGA1471050.019.60683814-15
AGAGTAC1099900.019.59800318-19
ACGACGC200600.019.1068825
AACGCAG1566950.018.00064814-15
CAGTGGT2350550.017.151684
TCCGATC458400.016.5530286
CGCAGAG1649550.015.71548416-17
TATCAAC1625300.014.29624210-11
GCAGTGG3486850.013.46647553
Produced by FastQC (version 0.11.5)