FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences350729007
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCGTTCGTTATCGGAATTAACC5767400.16444034810043526No Hit
AAGCCGCAGGCTCCACTCCTGGTGGT3832410.1092698329340065No Hit
ACGCCAGAGTCTCGTTCGTTATCGG3657170.10427338278296439No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAATGA1320100.018.2867551
CTCGAAA1056700.018.2092761
GAATGAA1222950.018.0959011
GACTAAC1087950.017.8637921
AAGAAGG1029950.017.8622767
CGCTTCA1938250.017.798141
GCGCGAT1105750.017.7760091
TACGGAT1632150.017.749351
CAGCATA1839400.017.6915111
CTAATGG1778600.017.5995181
CAATGAA787250.017.5861178
GAACCTA1050100.017.549231
AGAAACC1567300.017.5144218
CTACATT2634400.017.5027541
TGACAAC1528400.017.495241
GATGAAA753250.017.48721
ACGAGCC1628850.017.469671
TACCTTA2040950.017.455381
AGACTAG1005450.017.413298
CGACCTT1902050.017.4077341
Produced by FastQC (version 0.11.5)