FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences350729007
Sequences flagged as poor quality0
Sequence length8-98
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1587600.040.4392979
GGTATCA1750350.035.8527878
AAGCAGT2275150.035.342881
TGGTATC1889350.031.1483577
ATCAACG1698750.029.7760510-11
CAACGCA1909250.028.3365512-13
TCAACGC1806900.026.00322312-13
AGCAGTG3104450.025.3484022
ACGCAGA2284550.023.4704914-15
CTACACG255250.023.3560541
AACGCAG2218550.022.7604414-15
AGAGTAC1617200.022.61452318-19
ACGACGC278000.021.1250175
CGCAGAG2359900.020.15856716-17
GTGGTAT3214650.019.061976
TACACGA333450.017.9032342
GCAGTGG4521250.016.1451193
AGTGGTA4016850.015.6903255
CAGAGTA2274450.015.58445518-19
GAGTACT2037000.015.30490220-21
Produced by FastQC (version 0.11.5)