FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences359418640
Sequences flagged as poor quality0
Sequence length98
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1691400.043.280019
GGTATCA1946500.036.277778
TGGTATC1972100.034.6473167
AAGCAGT2484450.033.5293461
GTGGTAT2173200.031.6158226
ATCAACG1771250.028.541810-11
CAACGCA1950450.027.36299312-13
TCAACGC1843900.025.13050512-13
AGCAGTG3298150.024.2271082
ACGCAGA2267000.023.65150314-15
CAGTGGT3368450.020.9217034
AGAGTAC1923400.020.5190718-19
ACGACGC297300.020.4341835
AACGCAG2485950.020.36588914-15
CTACACG323650.019.154281
CGCAGAG2532950.018.90797416-17
GCAGTGG4232300.018.1833953
GCAGAGT2522800.016.29222516-17
CAGAGTA2367750.016.07394418-19
TACACGA394950.015.5161262
Produced by FastQC (version 0.11.5)