FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences359106732
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAAAGC1276750.018.2044371
ATAAGAG1823750.017.8772541
CCGGGAT1315850.017.7860771
GGGACCT1369400.017.781381
ACGAGGA995850.017.686371
AGAAAGG1111350.017.6783710
GTGATAA1047000.017.6510858
ATAACAA778300.017.6394654
TATATGA905550.017.5486899
AATAACA839200.017.5475273
CTCGAAA676800.017.5159441
ACTGAAC1026100.017.4581381
CAATGAA704300.017.4257018
TGATAAA950750.017.4043679
ACAATAG1115300.017.3990367
AAGACTA791450.017.3695817
AGATACA842900.017.3524788
GCAGAGA642650.017.345794
AGTGAAG1251550.017.3295658
ATAGACC958000.017.3274291
Produced by FastQC (version 0.11.5)