FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences359106732
Sequences flagged as poor quality0
Sequence length8-98
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1384450.037.023619
GGTATCA1579250.031.0886868
ATCAACG1444050.028.1690410-11
TGGTATC1695400.027.4095847
AAGCAGT2443200.027.3011761
CAACGCA1687250.026.55842212-13
TCAACGC1610100.023.6991512-13
GTGGTAT2053450.022.706316
ACGCAGA1975250.022.19589814-15
CTACACG267300.021.6344761
AGCAGTG3006600.021.0662692
AACGCAG2001550.020.77764114-15
ACGACGC292350.019.3498825
AGAGTAC1535600.018.96900718-19
TACACGA319500.017.7807042
CGCAGAG2307000.016.97241616-17
CAGTGGT2948600.016.8289814
AGTGGTA3342450.015.03352455
GCAGTGG4080800.014.5299223
GCAGAGT2161450.013.9936816-17
Produced by FastQC (version 0.11.5)