FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369583009
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGAT1254400.018.1275221
GAATGAA1129250.017.9560851
GGCGACT1308150.017.9487231
CAATGAA1001050.017.9239168
AACGAAA1025800.017.90704510
ACTGAAC1320450.017.903891
CGGAGCT1568400.017.5882171
CTCGAAA751350.017.500321
CAGAAAG903250.017.4851363
ATAGACC955350.017.3440651
AAACCTG1494950.017.2429371
CAATGGA1425100.017.154268
TCTAACT1679100.017.1464468
CAACGAA1087250.016.9514339
CTGAAAC940000.016.9260371
GTGATAA666600.016.9225858
ATGAATG954300.016.79805210
GATGAAA609000.016.7665271
TCGAGGC1454650.016.7448271
GCCGAAG660250.016.703173
Produced by FastQC (version 0.11.5)