FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369583009
Sequences flagged as poor quality0
Sequence length8-98
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1187650.032.9837469
ATCAACG1156850.027.74970210-11
AAGCAGT2151600.026.057651
CAACGCA1400000.025.78716912-13
GGTATCA1497900.025.6294828
TCAACGC1273950.023.74252312-13
GTGGTAT1579250.023.1530426
ACGACGC251850.022.6638135
TGGTATC1707400.021.5360057
ACGCAGA1682250.021.1067314-15
AACGCAG1792650.018.93221714-15
AGCAGTG3038250.018.2144282
AGAGTAC1264650.018.2141818-19
CGCAGAG1907650.016.71204616-17
CACGACG350300.016.341154
CTACACG402050.014.4919841
CAGTGGT2952150.013.4118784
GCAGTGG3841750.013.061943
TACACGA455400.012.5574532
AGTGGTA3291750.012.4285945
Produced by FastQC (version 0.11.5)