FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367410974
Sequences flagged as poor quality0
Sequence length8-98
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15342340.4175797971674085No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA3143050.060.431059
GGTATCA3466600.054.789828
TGGTATC3580450.051.9734957
AAGCAGT4465000.046.9395681
GTGGTAT4011600.046.4198726
AGCAGTG4673900.045.192542
CAGTGGT5104400.037.721824
GCAGTGG5822850.036.247573
ATCAACG3279100.035.6601510-11
CAACGCA3682500.034.0843412-13
AGTGGTA5692200.033.796335
ACGCAGA3689900.032.93670314-15
TCAACGC3487900.031.88003212-13
AACGCAG3781250.031.00741214-15
CGCAGAG3880500.029.26716416-17
AGAGTAC3453050.029.16036818-19
GCAGAGT3957850.026.13536516-17
TATCAAC3805700.025.66446710-11
GTACTTT3361650.025.4610422-23
CAGAGTA3865850.024.7525218-19
Produced by FastQC (version 0.11.5)