FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences365892371
Sequences flagged as poor quality0
Sequence length98
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1526750.039.56589
AAGCAGT2201050.032.6431921
GGTATCA1865850.031.766258
GTGGTAT2089050.027.4343726
ATCAACG1593200.027.18817910-11
TGGTATC2171550.026.3200877
AGCAGTG2822700.024.7008692
CAACGCA1854500.024.68707312-13
TCAACGC1653400.024.13340612-13
AACGCAG2121950.020.35093114-15
AGAGTAC1654600.020.2147818-19
ACGACGC286000.020.0535555
ACGCAGA2289700.020.04505314-15
CAGTGGT3051850.019.2563884
CTACACG322600.018.63581
CGCAGAG2295100.017.82339916-17
AGTGGTA3572200.017.1845785
GCAGTGG4157700.015.9974263
TATCAAC2206750.015.21775710-11
TACACGA396600.014.9772012
Produced by FastQC (version 0.11.5)