FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378488679
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGGCTGTTAATTGTCAGTTCAGTG6935230.18323480687251942No Hit
GCTGCTTTTAGGCCTACTATGGGTGT5700340.15060793931963287No Hit
ACGCCGAGTTCCTTTTACTTTTTTT4317740.11407844513098368No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGAA1395400.017.8461258
ATAGACC1143800.017.7160431
ATAACGA1412100.017.69620710
ACGCCGA2316300.017.5704291
TGGACGC1744500.017.419061
AATTGGA1097500.017.34114310
GACCAAT1588650.017.2957321
GCCGAAG1189750.017.1804313
GTCCGAA1594700.017.1179588
CGCCGAA1064450.017.0220182
CTCGAAA972050.016.9866711
TTCGTAA1050550.016.9761545
CTGCCTA1307000.016.961731
TAGTTGG1783200.016.9323481
GGGAGAT1007400.016.9247191
TCAATGA1093700.016.8723727
CAATAAC1488500.016.8320268
GATCTAG1725950.016.8209111
CGTTGGG2590100.016.8049241
CCTTACG1607450.016.800331
Produced by FastQC (version 0.11.5)