FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378488679
Sequences flagged as poor quality0
Sequence length8-98
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10686620.2823497925548256No Hit
GGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTA4233450.11185143003973443No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA2215200.061.0381249
GGTATCA2464850.054.8179638
TGGTATC2756050.048.192917
GTGGTAT2934850.045.4235046
AAGCAGT3739600.039.4708941
AGCAGTG3886350.038.137972
CAGTGGT3609450.037.7860454
ATCAACG2188000.037.27744310-11
CAACGCA2459700.034.91835412-13
TCAACGC2399950.033.55150212-13
AGTGGTA4075550.033.243935
ACGCAGA2644550.031.42760514-15
GCAGTGG4730400.031.3449213
AACGCAG2712500.030.70021614-15
CGCAGAG2712300.029.55971716-17
AGAGTAC2448300.028.00711618-19
GCAGAGT2843650.024.90016716-17
TATCAAC3010200.023.44809310-11
CAGAGTA2872950.023.31707818-19
GTACTTT2626100.023.04675722-23
Produced by FastQC (version 0.11.5)