FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360875445
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCGTTCGTTATCGGAATTAACCA4445050.12317407741610129No Hit
AGGCCGTATCGTTCCGCCTGGGCGGG3647690.10107891934847493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAC1149150.018.3251971
GAATGAA1004900.017.9624751
CTCGAAA812450.017.9536441
CTGCCTA1904750.017.8842531
GCGCGAT880550.017.75281
TCTGGAA1193650.017.4988251
ACTAGAC918850.017.4878645
GGGCATC1398450.017.476871
ACTGATG1107650.017.4613291
CCAGCGA1108300.017.4437871
CAATGAA717950.017.4410768
GGGAGAT927550.017.4137131
CGCTGGA1176600.017.3630031
GCCTCTA1899800.017.3473681
AAGAAGG709550.017.236137
AGAGCGA1090750.017.1708831
CTAATGG1227200.017.1663061
CAATGGA1328250.017.1635388
CAATAGA961550.017.1552098
TATAAAC849050.017.1486349
Produced by FastQC (version 0.11.5)