FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366685806
Sequences flagged as poor quality0
Sequence length8-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGAG940150.018.5310635
GAAAAGA816300.018.4499684
GATGAAA1086100.018.0430811
ATCGAGT1421750.017.7580681
AGAAAAG551650.017.713783
AAGAAAA551150.017.7108862
ACGGAGA1340150.017.5924681
AGGACGA637400.017.4741548
GGCTCGA1260050.017.3925111
CTCGAGG1295250.017.316331
GGAAAGC900050.017.288311
CACCGAA651750.017.2766958
GGACGAA644950.017.2665529
ATGGGAG1280200.017.2460421
ACTGAAC871200.017.1999341
GGATTAC1385800.017.0343281
GATCTAG1477600.016.9685521
CAATGGA1090800.016.958278
GAAATGA882150.016.946131
CCGGGAT999800.016.9157261
Produced by FastQC (version 0.11.5)