FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366685806
Sequences flagged as poor quality0
Sequence length8-98
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12094480.329832237902331No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA2320500.063.4702039
GGTATCA2595450.056.6527758
TGGTATC2676300.053.8759357
GTGGTAT2937250.049.3637126
AAGCAGT3671500.043.6952741
AGCAGTG3738800.043.1686632
CAGTGGT3880700.038.566624
ATCAACG2351550.037.6846810-11
CAACGCA2624000.035.51100512-13
TCAACGC2554050.034.01687212-13
GCAGTGG4903100.033.037323
ACGCAGA2745200.032.94233714-15
AGTGGTA4601450.032.4394575
AACGCAG2800250.032.23343314-15
CGCAGAG2945600.029.400316-17
AGAGTAC2693400.027.8469318-19
GTACTTT2572850.025.76686122-23
GCAGAGT3032650.025.45623416-17
CAGAGTA2933700.024.9762318-19
TATCAAC3152600.024.33788710-11
Produced by FastQC (version 0.11.5)