FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388436636
Sequences flagged as poor quality0
Sequence length8-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCGTTCGTTATCGGAATTAACC9808300.2525070781428557No Hit
CGTTCGTTATCGGAATTAACCAGAC4853340.12494547501950871No Hit
AAGCCGCAGGCTCCACTCCTGGTGGT4680360.12049223904822406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAA1453250.018.272371
GATGAAA1145550.018.1105611
ATGCGAT1512700.017.8877121
GAATGAA1092050.017.814971
GGGAGAT1425700.017.7499661
GGCTCGA1358100.017.664881
CCTCTGA2209900.017.5425361
TGAGCCG1464100.017.4843851
CAATGAA776900.017.3226628
TTAGGCA1236700.017.2795851
GGGCATC1543850.017.2481141
GAATAAG1184550.017.2479341
ATAACGC1575600.017.2420351
TGCGAAA725800.017.1305839
GTGATAA932650.017.109988
CGCTTCA1720800.017.1010841
AAACCTG1300850.017.0592861
AGGCCGT1969950.016.995721
GAAATGA1066550.016.9203661
AGGGTTA1264650.016.8823999
Produced by FastQC (version 0.11.5)