FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388436636
Sequences flagged as poor quality0
Sequence length8-98
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA879850.032.2334639
GGTATCA1149400.024.4699788
ATCAACG806100.021.93270110-11
CTACACG275300.021.0319251
TCAACGC802050.020.6085612-13
ACGACGC302400.018.704075
CAACGCA999000.018.34637312-13
TACACGA355600.016.0299972
AGAGTAC986300.014.9727118-19
AGTGGTA2053200.014.3377725
AAGCAGT2363850.013.7775671
ACGCAGA1413400.013.40309114-15
AGCAGTG2484300.013.1331122
CAGTGGT2275100.012.9678294
GTGGTAT2192950.012.9399126
TGGTATC2245700.012.5894547
ACGCTCT509550.011.1472538
CGCAGAG1756700.09.86683216-17
AACGCAG1859700.09.82476714-15
ACACGAC629500.09.1836453
Produced by FastQC (version 0.11.5)