FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences396045144
Sequences flagged as poor quality0
Sequence length8-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTCCGTCTTGCGCCGGTCCAAGA6062510.15307623617776261No Hit
AAGCCGCAGGCTCCACTCCTGGTGGT5095340.12865553528917906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACCT3890250.018.2447551
TGAGCCG1829350.018.0378441
TGACTAG1766350.017.9616741
AGGTCCG3708500.017.901481
GGCGACT1815800.017.7569681
CGACCTT2388800.017.7489241
CATAGAA1176600.017.7315678
AACGAAA837850.017.71954210
GGGAGAT987400.017.711811
GACTAAC1024500.017.592951
CAATGGA1744500.017.564758
AGGGTGA2202300.017.5301571
GTCCAAA1242300.017.5260497
GCCTCTA2377150.017.4614071
ATGGGAG1821650.017.460491
AGTCTAG1669500.017.3942247
CCGCGAG1243000.017.3864213
GGGCATC2079100.017.3716281
CCTCTGA1772050.017.325991
CTGATAG1482150.017.2996121
Produced by FastQC (version 0.11.5)