FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences396045144
Sequences flagged as poor quality0
Sequence length8-98
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1110050.034.1372269
GGTATCA1303050.028.9209618
ATCAACG909800.025.61706210-11
TCAACGC902250.024.07421712-13
AAGCAGT2201900.019.4915161
CTACACG250050.018.2755371
CAACGCA1329350.017.9266712-13
AGAGTAC1105600.017.34833718-19
AGTGGTA2342500.016.6056255
AGCAGTG2865850.015.0970952
CAGTGGT2591150.014.9681294
GTGGTAT2541700.014.9134286
TGGTATC2669450.014.1294847
ACGACGC329000.013.8140295
ACGCAGA1906050.012.83791314-15
AACGCAG1928350.012.05812414-15
CGCAGAG1991350.011.33909416-17
TACACGA417000.011.0118552
GCAGTGG4068900.010.5894493
GAGTACT1778650.010.22502320-21
Produced by FastQC (version 0.11.5)