FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences387561046
Sequences flagged as poor quality0
Sequence length8-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCGTTCGTTATCGGAATTAACC4114140.10615463144353263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAA1827500.018.8912261
GAATGAA1823750.018.6943591
GAACCTA2008450.018.4532131
CCGGGAT1938100.018.353371
CAATGAA1194100.018.2435788
GAAATGA1362450.018.2323251
TACACGA1472250.018.2160971
GGAGCGA1348600.018.1202779
GCGCGAT1614500.018.1013091
CCCTCCT4904800.018.0233081
CTCAGAA1256700.017.7858811
GCGATCA1814200.017.6623693
TGAGCCG1697150.017.6563381
CCGCTAG1649100.017.63290410
CCGGAAA750750.017.63165510
AGACGTT1698750.017.6198841
TACGGAG1255400.017.6002810
GACTACA1249500.017.5784171
AATGGAA923550.017.577769
CACGCGA1183950.017.5614648
Produced by FastQC (version 0.11.5)