FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences387561046
Sequences flagged as poor quality0
Sequence length8-98
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA769500.025.3383249
ATCAACG601350.020.98963710-11
CTACACG281750.018.972961
TCAACGC667450.017.67677512-13
ACGACGC308000.016.7272665
CAACGCA818650.016.1331412-13
GGTATCA1250000.015.6885048
AGAGTAC830400.012.25904418-19
TACACGA455300.011.5767762
ACGCAGA1325250.010.25262214-15
AGCAGTG2193200.010.1518852
AGTGGTA2039650.010.0251765
AAGCAGT2243400.09.9212581
AACGCAG1398400.09.33511614-15
TAGCGCG568850.09.26806992
ACGCTCT578650.09.2672548
CACGACG614750.08.4058664
CAGTGGT2463350.08.1438994
CGCAGAG1585300.07.782833616-17
CTAGTCG569550.07.213745692
Produced by FastQC (version 0.11.5)