FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394072270
Sequences flagged as poor quality0
Sequence length8-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGGCTGTTAATTGTCAGTTCAGTG5283530.13407515327074396No Hit
AAGCCGCAGGCTCCACTCCTGGTGGT4552100.11551434461501185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAC1245300.018.0289081
GAATGAA1064350.017.8100871
AGAAACC1461350.017.7869078
CTCGAAA922650.017.7564331
CTAAGAC1291800.017.7337511
CACCGAA1001050.017.6290458
GAATAAG1024800.017.6276341
CAATGGA2529650.017.5773568
GAAGAAA595000.017.4586586
GAAATGA873300.017.279221
GATGAAA717700.017.1919651
GACCAAT1344150.017.1851331
GGGCATC1671050.017.1629181
CGTTAGA1242950.017.1572171
CCGGGAT1058600.017.1395661
AGGGTGA1757100.017.072931
GCCGAAG718550.017.0708453
ACGCCGA1641800.017.0631561
ACGGGCT3354550.017.029031
CCACTAC1678350.017.0228391
Produced by FastQC (version 0.11.5)