FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005798495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005798495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394072270
Sequences flagged as poor quality0
Sequence length8-98
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA723300.030.6328249
GGTATCA992200.022.289688
ATCAACG712100.019.69232410-11
TCAACGC736950.018.15928812-13
ACGACGC362100.017.5310615
CTACACG372850.017.222811
CAACGCA976000.014.86513712-13
TACACGA456700.013.9514992
AGAGTAC934800.011.96782818-19
ACGCAGA1269800.011.77264814-15
AGTGGTA2041650.011.6927475
AAGCAGT2290150.011.6121611
ACGCTCT581650.011.1108978
TGGTATC2048650.010.7841937
GTGGTAT2104500.010.6109996
CAGTGGT2234200.010.454834
AGCAGTG2582650.010.2284342
CACGACG678100.09.3220924
ACACGAC692850.09.3218253
AACGCAG1616200.09.05259114-15
Produced by FastQC (version 0.11.5)